Medicinal plants growing in Mt Prau, Central Java, Indonesia were surveyed, and their traditional uses and preparation methods were documented. The entire study area was stratified into three zones based on altitudes, and a total of 23 medicinal species were identified and recorded. Tetrastigma glabratum (Blume) Planch was the main herbal used in Mt Prau for at least three generations. The exudate from large stem is believed to cure several ailments and diseases. T. glabratum was found in small numbers at >1300m above sea level and was not found in the lower altitudes. Preliminary phytochemical analysis in T. glabratum plant extracts confirmed the presence of diverse group of phytochemicals (steroids, terpenoids, flavonoids, saponins, and tannins). There is a need to conserve T. glabratum, and recommendations are made.