The purpose of this study is to explain how high reading comprehension proficiency of Biology Education Students, to explain how high the reading comprehension proficiency of Siyasah Jinayah Students, and to explain the proficiency differences in reading comprehension between Biology Education Students and Siyasah Jinayah Students. The research design was comparative study used the Test strategy, after collecting the data, it was found that the mean Biology Education Students score was = 71.355, and the mean score of Siyasah Jinayah Students = 71,871. Meanwhile, the mean score of Try Out English Education Students is 72,129, English Education is here as an mean comparison reference on reading comprehension and English Education here as a representative of English Language Course which is in the third semesters which is on Biology Education Students and Siyasah Jinayah Students. Based on the results of the study, the researcher concluded that the mean of reading comprehension on proficiency is little difference between Biology Education Students and Siyasah Jinayah Students at the Third Semesters UIN Walisongo Semarang in Academic Year of 2017/2018.