The abundance of coconut plants in Indonesia which is in contrast with the price of oil, demands research that can bridge the problem, namely the process of making coconut oil which implements procedures that are easy, inexpensive and with low energy. This study aims to determine the comparison of the results of coconut oil obtained with the help of Crustachea animal substrate. The research method was carried out experimentally, each sample had three replications. Ingredients in the form of coconut each 300g plus substrate from animals Crustachea (100gr, 75gr, 50 gr, 25gr, and control 0 gr). The volume of oil formed between 100-150 ml. The coconut oil formed was each tested, first organoleptically, obtained by a fragrant and slightly fishy aroma. Furthermore testing with various measuring instruments; 1) freezing point test was carried out at 5 C, a significant result was obtained on the crab substrate. Crab freezes longer than crab, which is 45 minutes and crab 30 minutes;; 2) free fatty acid (FFA) levels were tested using the titration method and the results obtained, crab (Scylla serrata) 6.232 ml / L and crab (Portunus pelagicu) 24,598 ml / L. Based on data from FFA (Free Fatty Acid) content, oil with crab substrate has good potential for health due to low FFA levels, compared to oil from small crab substrate.