Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines
Lianah, Lianah and Khasanah, Rita Ariyana Nur and Pranatami, Dwimei Ayudewanda and Krisantini, Krisantini (2021) Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines. Biodiversitas, Journal of Biological Diversity, 22 (2). pp. 913-919. ISSN 1412-033X (print); 2085-4722 (online)
jurnal Phyto no 2.pdf - Published Version
Download (610kB)
Abstract
The aim of the study was to analyze the phytochemical contents and todetermine the cytotoxic activities of Bauhinia scandens leaf ethanol extracts against HeLa (human cervical cancer) and T47D (breast cancer) cell culture lines. Using a standard protocol, it was found that leaf extract of B. scandens contains phytochemicals that are widely known to have medicinal properties, such as phenols (17.57±0.098% w/w), flavonoids (5.91±0.098% w/w), saponins (19.42±0.091% w/w), tannins (1.24±0.035% w/w), alkaloids (1.31±0.001% w/w) and steroids (0.08±0.007% w/w). Results of 24-hr Microculture Tetrazolium Salt (MTT) assay show that leaf extracts of B. scandens can suppress cell growth at a half-maximal inhibitory concentration (IC50) of 1.95 μg/mLfor HeLa cells and 4.54μg/mL for T47D cells. This indicates that B. scandens is a potentially strong candidate for anti-cancer agents. The cytotoxic activities of the leaf extracts of B. scandens can be attributed to the total effects of the phytochemical compounds found. Further studies are recommended in order to isolate and determine individual effects of bioactive compounds in B. scandens.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Bauhinia; Cytotoxicity; HeLa cells; Phytochemicals; T47D cells |
Subjects: | 500 Natural sciences and mathematics > 540 Chemistry and allied sciences > 547 Organic chemistry |
Divisions: | Fakultas Sains dan Teknologi > 46201 - Biologi |
Depositing User: | Miswan Miswan |
Date Deposited: | 01 Mar 2022 23:06 |
Last Modified: | 01 Mar 2022 23:06 |
URI: | https://eprints.walisongo.ac.id/id/eprint/15483 |
Actions (login required)
Downloads
Downloads per month over past year