A study was conducted to determine the growth and the best method of cutting propagation of Tetrastigma glabratum (Blume) Planch, a host of Rafflesiaceae. Rafflesia is the largest and magnificent flower in the world that requires growing parasitically on the host plant. T. glabratum was found to grow in the naturally protected rainforests at Mount Prau, Candiroto, North Kedu, Central Java, Indonesia. T. glabratum existence is threatened due to illegal harvesting by the local people who use liquid exudates from the mature stems as an energy drink and to cure various diseases. The population of T. glabratum is increasingly hard to find, particularly the mature plants. Ex situ conservation and cultivation is very important to protect T. glabratum. Propagation studies were conducted using stem cuttings and treated with IBA at 500, 1000, 2000, and 4000 ppm, and a commercially available rooting hormone containing naphthalene acetic acid (NAA) versus non-treated as a control, and through air layering. In the second experiment, stem explants were cultured in vitro using MS media. IBA and NAA increased the percentage of survived and rooted cuttings over control, and IBA at 2000 ppm significantly improved the percentage of rooted cuttings (50%) compared to 5% without IBA. Air layering techniques had more success than cuttings, with tree fern media giving 70% success rates with the highest number and longest roots. In vitro methods have been developed to obtain sterilized explants from the mature plants growing in situ but have not been successful to induce proliferation even though the explants had started showing enlargement around the nodal section.