Learning in the 21st century creates real changes in the world of education that focus on the learning process. One of the skills that students must have in the 21st century is the skill to think creatively. However, the results of the measurement of creative thinking skills that researchers conducted at SMAN 16 Semarang in August 2023 using human circulatory system material showed that students at SMAN 16 Semarang had low creative thinking skills as evidenced by the results of the creative thinking skills test on 3 indicators, including fluency indicators had a percentage of 40%, flexibility indicators had a percentage of 38%, and originality indicators had a percentage of 22%. The learning model that can develop creative thinking skills is the Project Based Learning model based on 3D media. The purpose of this study is to analyze the effect of Project Based Learning model based on 3D media on creative thinking skills in biology learning. The novelty in this research is using the 3D media-based Project Based Learning model. The type of research used in this study is quasiexperiment with Nonequivalent Control Group Design. The sampling technique used was Random Sampling with samples of class XI.1 as the experimental class and class XI.3 as the control class. The data collection techniques used in this study were interviews, observations, documentation, and pretest-posttest sheets about creative thinking skills. The results showed that there was an effect of 3D media-based Project Based Learning model on creative thinking skills in biology learning, this can be seen from the One Way Anacova test analysis of creative thinking skills with a significance value of 0.000 <0.05.