In the initial survey conducted by the researchers found that villagers had exploited growing Blumah Tetrastigma glabratum or name of the region alley walikadep started since time immemorial very day. This resulted in the existence of exploitation Tetrastigma glabratum plants in their natural habitat in protected forests Prau mountain in Central Java. If exploitation is not addressed, then this plant will be threatened depleted or become extinct. In terms of ecology, when a species becomes extinct then it will have an impact on the extinction of other species. In addition, the presence of these plants in their natural habitat is also influenced by external factors such as floods, erosion, landslides and forest firesbjective To determine the effect of several concentrations of hormones IAA (indole butyric acid) Yates clonex to root growth and root formation process plant Tetrastigma glabratum. The Method pure experimental research. Each treatment plant propagation T. glabratum who had let the treatment of propagation on the rod smeared / dosed, respectively 500 ppm, 1000 ppm and 2000 ppm. wrapped with planting medium. Each sample 3 repetitions control without hormones. A planting medium: sand, soil, ferns and Jeffy powder and mixed media, and then tied up and labeled left for 3 weeks. Total propagation experiment is each dose of 5x3 replicates x 4 kinds of media. Number 60 stem propagation experiments. Based on the results of the study showed that a dose of 2000 ppm IBA most root growth and the least number of roots at a dose of 500 ppm. Average of 5 planting medium from the finest powder ferns.