This study investigate language variety in English class that employed by male and female students. It explores male and female language variety as affected by gender and culture. The data collected in 2020 from 5 males and 5 females students of MTs Darul Ulum Ngaliyan. It also reveal male and female student’s character that affected by gender differences which also affected their language variety. This study employed a descriptive qualitative method. Which the data were obtained through observation and interview and analyzed using interactive analysis model by Miles and Huberman. The result of this research showed male and female language varieties of eighth grade students of MTs Darul Ulum Ngaliyan. It is revealed that male students have a dominant and competitive attitude as indicated of masculine culture. Male students also dispossed to use straightforward, attention-commanding and definitive language expression. Whereas, female students exhibit commodating and negotiative character that identical to feminine culture that is a. So their choice of languages is in line with variations in female's languages. Their thypical female students language variety, for example is frequently using question tags, qualifiers, and change statements to question.