Listening strategy is one of the most important factors that can influence the listening comprehension process. Using the right strategy is required to make it easy for students to grasp what the speaker is saying. Regarding its relation to gender, gender has been identified as one of the main variables that can influence how students use listening strategies. This research aims to analyze the degree of the difference in the use of listening strategies between male and female learners. The researcher employed a quantitative research approach. The population was 191 fourth-semester English Education Department students of UIN Walisongo Semarang in the academic year 2022/2023. Using the Isaac and Michael formula, the sample size of 191 populations with an error of 5% is 128. Using cluster sampling, the sample is determined for male and female groups, 31 male and 97 female learners are selected as the sample. Data was collected using a questionnaire which consists of 23 statements. Data were analyzed using a t-test of 2 independent samples, the result is 0.015 (p < 0.05), which indicates there is a degree of difference in the use of listening strategies between male and female learners. Male learners were categorized at a moderate level in using listening strategies. Meanwhile, female learners were categorized as moderate to high in using listening strategies. In metacognitive strategies, male learners got 3.32, and female learners got 3.70. In cognitive strategies, male learners got 3.31, and female learners got 3.48. And in social/affective strategies male learners got 3.24 and female learners got 3.44. It can be concluded that female learners used more listening strategies than male learners. Therefore, gender plays a role in determining the use of listening strategies.