This study aims to analyze the significance differences in speaking anxiety between male and female EFL students based on gender. This study looked closely at whether or not there are gender differences in speaking anxiety when using the English language. A descriptive quantitative research methodology was employed in this study. 50 undergraduate EFL students of UIN Walisongo in the academic year 2022/2023 were selected as the participants. The data were gathered through a questionnaire. Male and female students' levels of speaking anxiety were determined using simple calculations. The statistical Mann-Whitney U test was used to determine whether there were any differences between genders in terms of speaking anxiety. According to the results, male and female students exhibit different levels of speaking anxiety; nevertheless, further analysis using the Mann-Whitney U test reveals that the difference is not statistically significant, with a score of Asymp.Sig. (2-tailed) of 0.684 > 0.05. According to the research's findings, male and female students experienced similar anxiety levels when learning a foreign language. Based on data analysis performed using independent sample t-tests in SPSS software, they just barely differ from each other. Additionally, male and female students have their own speaking anxiety that is different from one another.